DNA and RNA Extraction
Two Decades Experience in Extracting DNA and RNA from a Wide Variety of Starting Material
BioServe has 20 years of experience with genomic DNA isolation from nearly all biomaterials including whole blood, blood clots, buffy coats, PMBCs, nuclear pellets, serum, saliva, mouthwash, buccal swabs, cytobrushes, plant material, cultured cells, fresh and frozen tissues and paraffin embedded tissues.
BioServe utilizes different types of reagents based on the type of biomaterials. The most common of which are our DNAQuik™ reagents developed by BioServe.
DNAQuik reagents are non-organic and yield good quality, high molecular weight genomic DNA. BioServe has utilized DNAQuik reagents for DNA extraction from a variety of tissues. BioServe also utilizes organic reagents to obtain high quality DNA from buccal swabs and cytobrushes.
Genomic DNA is quantitated by measuring optical density at 260 nm and A280 nm wavelengths. Samples that traditionally yield small quantity of DNA or have bacterial DNA are quantitated using Real-Time PCR using a probe specific for human DNA. BioServe also has capabilities to provide Picogreen quantitation.
Quality of DNA is determined by electrophoresing a small quantity of DNA (25 ng to 200 ng) on 1% agarose gel. BioServe typically performs quality check on 10% of the samples. In case of mouthwash, buccal swabs, cytobrushes, and paraffin embedded tissues, quantitation by Real-Time PCR also serves as a quality check. The quality of some DNA samples isolated at BioServe has been assayed after 15 years, and determined to be of high quality after storage in -70°C.
Genomic DNA isolated at BioServe has been utilized in several molecular biology and genomics applications. These include SNP analysis by MassARRAY (Sequenom), Masscode (Qiagenomics), Beadchip Array (Illumina), GeneChip Array (Affymetrix) and TaqMan (ABI).
Please contact us or visit our online presentations for specific details and protocols, as well as QC/QA assays.
BioServe has 20 years experience with RNA isolation from a variety of starting material. We have been successful in RNA extraction from whole blood, buffy coats, PAXgene tubes, lymphocytes, cultured cells, plant material, cartilage, fresh and frozen tissues and various bacteria.
BioServe utilizes reagents from Qiagen and /or RNAQuikTM reagents depending on the type of tissue. BioServe has developed protocols for RNA purification from tissues such as cartilage.
BioServe will provide A260/A280 ratio and concentration for RNAs isolated. The quality of RNA isolated is determined using Agilent's Bioanalyzer. RNA isolated at BioServe has been used in various molecular biology and genomics applications. These applications include expression analysis using RT-PCR, micro array analysis using Affymetrix GeneChip system and library construction.
To obtain high quality of RNA BioServe recommends that the samples should be immediately cryo-preserved upon collection and properly shipped to BioServe. Sample sets received for RNA extraction are logged in, entered into a database, and stored at -70°C until processing. After RNA extraction is completed, samples are frozen at -70°C, packaged with a data sheet and Bioanalyzer data and shipped to the customer.