BioServe Genotyping Services

The Genotyping Department of BioServe combines state-of the-art platforms with years of genotyping experience. Our in-house capabilities in assay design, oligonucleotide synthesis and SNP validation enable us to deliver accurate data, quickly and cost-effectively. We specialize in smaller to mid-size projects, providing intense customer service from assay design to data delivery and discussing your experiment results. By partnering with BioServe for your genotyping projects, you can expect:

1. Expert Assay Design: Choose from our pre-designed panels or have us design a variety of custom assays for your project
2. Fast Delivery Times: Most data are delivered within 5 days of receipt of properly quantitated and plated samples.
3. Data Analysis Assistance: Interpret your data easily with our intuitive data reports
4. Strict Quality Control Measures: All of our assays are validated in duplicate using 23 pairs of high quality "Polymorphism Discovery Resource" DNA from Coriell. We include up to three of these controls of known genotypes in analysis with customer samples (if plated according to our templates), as well as three negative (no DNA) controls. All control data are provided to the customer at no additional charge.

* Delivery times can vary depending on project sizes and requirements

Depending on your project details and requirements, BioServe™'s Genotyping Department will use one of the following technologies:

1. MassARRAY iPLEX (MALDI-TOF): Sequenom™'s most recent development in genotyping. The iPlex sample preparation enables increased multiplexing capability, high call rates, very high accuracy and increased sample throughput.
2. MassARRAY hME (homogeneous mass extend MALDI-TOF): Sequenom's initial genotyping platform. The robust platform is ideal for certain SNP assays which are not amenable to high plexing levels. This can occur when SNPs are surrounded by highly repetitive sequences, or have other difficult assay design issues. Although the multiplexing level achieved by the MassARRAY hME platform is lower than the MassARRAY iPLEX platform, the MassARRAY hME also provides high call rates, very high accuracy, and a high sample throughput.
3. Additional Platforms: Several molecular biology platforms can be ideal for certain types of genotyping projects,including non-SNP genetic polymorphisms (hME, TaqMan, PCR, DNA sequencing). These platforms include capillary DNA sequencing (useful for repeat polymorphisms), PCR based gel assays (detection of gene insertions and deletions), PCR-RFLP assays (to determine SNPs in regions of highly homologous gene families), and TaqMan (a lower throughput solution to some SNP assays).

Please contact us at 301-470-3362 or at genotyping@bioserve.com for more information on how BioServe can best assist you with your genotyping projects.