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MALDI-TOF MS-hMEThe principle and processing using the MALDI-TOF MS hME is same as that is for the MALDI-TOF MS iPLEX. The only difference being that MALDI-TOF MS hME uses traditional dideoxy nucleotides in the mass extension reaction. This could result in one or two base incorporation to discriminate between the two alleles. This also results in non-symmetric signals for the two alleles making it difficult to achieve higher plex levels. This method can be utilized and is sometimes preferred to iPLEX in determining repeat polymorphisms such as UGT1A1. BioServe has designed and validated this assay to analyze several sample sets. |
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