RNA Extraction Services

BioServe has over 15 years experience with RNA isolation from a variety of starting material. We have been successful in RNA extraction from whole blood, buffy coats, PAXgene tubes, lymphocytes, cultured cells, plant material, cartilage, fresh and frozen tissues and various bacteria. BioServe utilizes reagents from Qiagen and /or RNAQuikTM reagents depending on the type of tissue. BioServe has developed protocols for RNA purification from tissues such as cartilage.

BioServe will provide A260/A280 ratio and concentration for RNAs isolated. The quality of RNA isolated is determined using Agilent's Bioanalyzer. RNA isolated at BioServe has been used in various molecular biology and genomics applications. These applications include expression analysis using RT-PCR, micro array analysis using Affymetrix GeneChip system and library construction.

To obtain high quality of RNA BioServe recommends that the samples should be immediately cryo-preserved upon collection and properly shipped to BioServe. Sample sets received for RNA extraction are logged in, entered into a database, and stored at -70°C until processing. After RNA extraction is completed, samples are frozen at -70°C, packaged with a data sheet and Bioanalyzer data and shipped to the customer.