Direct Sequencing of PCR Products

PCR fragments submitted for sequencing should each be a single amplified fragment which has been analyzed on a PCR check gel (gel picture is requested). For best results with PCR fragments, we routinely use Clontech columns for purification. Results will be submitted with a hard copy of the sequence and electrophoretogram. Turnaround time for this service is usually 4-5 days.

When requesting this service, please provide the following:

• OPC® purified primers for sequencing
• 500 ng of amplified product per sequencing reaction.
• gel picture of PCR product