Gene Walk Sequencing

Gene walk sequencing projects are tailored to fit the individual needs of each customer. This service can be performed on either one strand or both strands of DNA. Gene walks provide contiguous sequence data for the region of interest. The turnaround time for these projects varies depending on the size of the fragment to be sequenced. BioServe utilizes the Beckman CEQ™ 8000 Genetic Analysis System sequencer or GE Healtcare's MegaBace 1000M and primer walking strategy to sequence the DNA fragments. At the end of the project, BioServe provides the customer with an assembled contigious sequence, a list of primers used, sequence generated from each primer and electrophoretograms.

When requesting this service, please provide the following:

• 100 µg of CsCl or Qiagen purified DNA
• vector type and size
• Cloned fragment size
• Initial sequencing primers (if not universal)